Device

Part:BBa_K1137011:Design

Designed by: Matthew Deyell   Group: iGEM13_Paris_Bettencourt   (2013-09-23)


sRNA anti lac


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 153
    Illegal XhoI site found at 159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Sequence of the sRNA must match exactly the target sequence for silencing. Therefore we needed to sequence the available lacZ to design our sRNA. If users lacZ has any point mutations, silent or otherwise, silencing may not occur.


Source

gBlock Synthesis with sRNA design from "Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli" Yoo et al 2013 Nature Protocols

References